Fig. 4

SlMED21 interacts with SlHUB1 but did not affect the resistance to B. cinerea. a SlMED21 interacted with SlHUB1 in yeast two-hybrid assay. Yeasts carrying the SlMED21 in the prey vector and the SlHUB1 in the bait prey vector were assayed for growth on selective medium (SD/Leu⁻ Trp⁻ Ade⁻ His⁻) and β-galactosidase activity after addition of X-α-Gal. The positive control pGADT7-T + pGBKT7-53 and other indicated combinations between empty vector and SlHUB1/SlMED21 were assayed in parallel. b Silencing efficiency of SlMED21 in VIGS construct-infiltrated plants. The silencing efficiency was calculated by comparing the transcript levels of SlMED21 in pTRV2-SlMED21-infiltrated plants to that in pTRV2-GUS-infiltrated plants, which were set as 1. c Disease symptom on detached leaves at 3 dpi. d Disease phenotype on whole plants at 6 dpi, respectively. e Lesion sizes on selected leaves in detached leaf inoculation assays at 3 dpi. Lesion sizes were measured on a minimum of 30 leaves in each experiment. f Growth of B. cinerea in inoculated plants from the whole plant inoculation experiments at 3 dpi. Relative fungal growth was shown as folds of transcript levels of BcActin compared to SlActin. Data presented are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level