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Fig. 9 | BMC Plant Biology

Fig. 9

From: Functional characterization of DnSIZ1, a SIZ/PIAS-type SUMO E3 ligase from Dendrobium

Fig. 9

Heat shock-induced accumulation of SUMO conjugates in transgenic lines. Total proteins were extracted from untreated (22 °C, 30 min) or heat-shocked (42 °C, 30 min) 10 d-old seedlings of the wild type (Col-0), siz1-2 (atsiz1-2), and each of transgenic lines (HB4, HB17). 20 μg of proteins was on an SDS-PAGE gel and the immunoblot was probed with an anti-SUMO1 antibody

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