Fig. 9From: Functional characterization of DnSIZ1, a SIZ/PIAS-type SUMO E3 ligase from Dendrobium Heat shock-induced accumulation of SUMO conjugates in transgenic lines. Total proteins were extracted from untreated (22 °C, 30 min) or heat-shocked (42 °C, 30 min) 10 d-old seedlings of the wild type (Col-0), siz1-2 (atsiz1-2), and each of transgenic lines (HB4, HB17). 20 μg of proteins was on an SDS-PAGE gel and the immunoblot was probed with an anti-SUMO1 antibodyBack to article page