Fig. 5

Subcellular localization of DnSIZ1 protein. a Schematic representation of constructs for constitutive expression in Arabidopsis root cells. CaMV35S, cauliflower mosaic virus 35S promoter; EGFP, green fluorescent protein; T _Nos , nopaline synthase gene terminator are indicated. b DnSIZ1 amplification and identification of transgenic Arabidopsis lines. The plasmids were transformed into Arabidopsis wild-type using an A. tumefactions-mediated floral dip method. c Co-expression of GFP fused to DnSIZ1. 35S::DnSIZ1:GFP #4 and 35S:GFP transgenic Arabidopsis root cells were observed, respectively. Signals were visualized using a confocal laser scanning microscopy. Panels from left to right: GFP fluorescence image; propidium iodide stained image; merged GFP fluorescence image. The GFP green color (Merged) revealed that DnSIZ1 is localized to the nucleus. Scale bars = 10 μm