Fig. 6

A single copy of the Sahara HvBot1 allele on chromosome 4H does not contribute to B tolerance in barley. a Semi-quantitative RT-PCR analysis of HvBot1 expression in roots of Clipper, Parent 19 and Ethiopia 756 seedlings. Each sample is derived from a single seedling grown in nutrient solution containing an additional 3 mM B for 15 days. The lower panel shows HvGAP fragment amplification, to indicate template concentration variability. + = positive control using HvGAP PCR product as template; − = water control. b Distribution of percentage leaf necrosis in F₂ progeny derived from the crosses Parent 19 × Clipper (left panel; N = 294) and Ethiopia 756 × Clipper (right panel; N = 337). Seedlings were grown for 15 days in nutrient solution containing 3 mM B. Average necrosis for each parent is also indicated. c Leaf necrosis of F₂ plants in the two populations segregating for HvBot1 allele (chromosome 4H; upper panels) and a KASP^TM marker linked to the 2H B tolerance locus (lower panels). In all panels, the Parent 19 or Ethiopia allele is represented by A, and the Clipper allele represented by B. The boundaries of the boxes indicate 75^th and 25^th centiles, lines within mark the median, bars above and below the boxes indicate 90^th and 10^th centiles, and outliers are shown as circles. The percentage variation (R²) explained in each population by marker genotype is also shown