Fig. 5

Contribution of Sahara-derived B tolerance alleles on chromosomes 2H and 4H to B tolerance traits in the barley cultivar Sloop Vic. a Root B concentrations (upper panel) and leaf symptoms of B toxicity (lower panel) in the parental genotypes Sloop and Sahara, and two Sloop Vic sublines. Subline Sloop Vic_A contains the multi-copy Sahara HvBot1 allele on chromosome 4H, while Sloop Vic_B contains the Sloop (Clipper) HvBot1 allele. Both sublines carry the 2H B tolerance allele from Sahara. Seedlings were grown for 13 days in solution culture (11 days at 3 mM B). Within each panel, italicised letters above the bars denote significant differences between genotypes (Tukey’s multiple comparisons test; P = 0.05) b Semi-quantitative RT-PCR analysis of HvBot1 gene expression in roots of Sloop, Sloop Vic_A, Sloop Vic_B and Sahara seedlings grown for 11 days at 3 mM B. Duplicate reactions were run for each of three cDNA samples for each genotype, and appear alongside each other in the image. The lower panel shows HvGAP fragment amplification, to indicate template concentration variability