Fig. 7

PEX5 degradation is reduced following treatment with the MG132 proteasome inhibitor. One-day stratification and one-day preincubation were performed prior to plating. Plates were placed in yellow light at 22 °C for one day and wrapped in aluminum foil at 22 °C for three days. Seedlings were moved to 0.5 % sucrose-supplemented liquid plant nutrient media with or without 50 μM MG132 in the dark for 24 h at 22 or 28 °C. Protein extracts of dark-grown seedlings were processed for immunoblotting. The membrane was serially probed with the indicated antibodies. Thiolase is synthesized as a PTS2-containing precursor (p) that is cleaved in the peroxisome to the mature (m) form. HSC70 was used to monitor protein loading. Band intensities were quantified using ImageJ. The levels of PEX5 and PEX7 were normalized to the corresponding HSC70 band prior to normalizing to the 22 °C non-MG132-treated wild-type band to give the listed numbers