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Fig. 6 | BMC Plant Biology

Fig. 6

From: Elevated growth temperature decreases levels of the PEX5 peroxisome-targeting signal receptor and ameliorates defects of Arabidopsis mutants with an impaired PEX4 ubiquitin-conjugating enzyme

Fig. 6

High temperature-induced PEX5 reduction in pex4-1 does not require autophagy. Plants were grown as in the legend of Fig. 1. Protein extracts of dark-grown seedlings from 0.5 % sucrose-supplemented plant nutrient media were processed for immunoblotting. The membrane was serially probed with the indicated antibodies. HSC70 was used to monitor protein loading. Band intensities were quantified using ImageJ. The levels of PEX5 or PEX4 were normalized to the corresponding HSC70 band prior to normalizing to the 22 °C wild-type band to give the listed numbers

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