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Fig. 5 | BMC Plant Biology

Fig. 5

From: Elevated growth temperature decreases levels of the PEX5 peroxisome-targeting signal receptor and ameliorates defects of Arabidopsis mutants with an impaired PEX4 ubiquitin-conjugating enzyme

Fig. 5

PEX5 is more membrane associated in pex4-1; high temperature does not rescue this defect. Extracts from dark-grown seedlings grown at 22 °C or 28 °C were cleared by a low-speed centrifugation to give homogenates (H). After a high-speed centrifugation, supernatants (S) were removed, and pellets were resuspended and spun at high speed to separate the wash supernatants (W) and final pellets (P). Equal volumes of each fraction were processed for immunoblotting by sequential incubation of the membrane with the indicated antibodies. PEX14 and mitochondrial (mito) ATP synthase are integral membrane proteins; HSC70 is mainly cytosolic. The positions of molecular mass markers (in kDa) are indicated on the left. Band intensities were quantified using ImageJ. For each sample, the supernatant, pellet, and wash were normalized to the corresponding homogenate to give the listed numbers

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BMC Plant Biology

ISSN: 1471-2229

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