Fig. 4From: Elevated growth temperature decreases levels of the PEX5 peroxisome-targeting signal receptor and ameliorates defects of Arabidopsis mutants with an impaired PEX4 ubiquitin-conjugating enzymeLow temperature worsens the peroxisomal defects of pex4-1. Seedlings were grown in the dark at 15, 22, 28, or 32 °C with or without 0.5 % sucrose (a) or 30 μM IBA (b). Means of normalized dark-grown hypocotyl lengths and standard deviation of the means are shown (n ≥ 20). c Protein extracts of dark-grown seedlings from 0.5 % sucrose-supplemented plant nutrient media were processed for immunoblotting. The membrane was serially probed with the indicated antibodies. Thiolase is synthesized as a PTS2-containing precursor (p) and cleaved in the peroxisome into a mature (m) form. HSC70 was used to monitor protein loading. The positions of molecular mass markers (in kDa) are indicated on the leftBack to article page