Fig. 3

Peroxisomal defects of pex4-1 are exacerbated by the pex5-1 and pex5-10 mutations. a, b Seedlings were grown as in the legend of Fig. 1. Means of normalized dark-grown hypocotyl lengths and standard deviations of the means are shown (n ≥ 7). No bars are shown for pex4-1 pex5-10 at 28 °C because of extremely poor germination rate (one seed germinated on 0.5 % sucrose-supplemented plant nutrient medium out of approximately 100 seeds plated; none germinated without sucrose or with 30 μM IBA). c Protein extracts of dark-grown seedlings from 0.5 % sucrose-supplemented plant nutrient media were processed for immunoblotting. The membrane was serially probed with the indicated antibodies. Thiolase is synthesized as a PTS2-containing precursor (p) and cleaved in the peroxisome into a mature (m) form. HSC70 was used to monitor protein loading. The positions of molecular mass markers (in kDa) are indicated on the left