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Table 3 Relative conversion of five alkaloids tested as potential substrates for six NMT candidates from Glaucium flavum

From: Transcriptome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants

Substrate

Relative activity (% maximum)

GFLNMT1

GFLNMT2

GFLNMT3

GFLNMT4

GFLNMT5

GFLNMT6

(S)-Coclaurine

100 ± 1a

nd

nd

100 ± 10d, e

<1

100 ± 14g

(S)-Reticuline

nd

nd

100 ± 5c

8 ± 1

100 ± 5f

nd

(S)-Canadine

nd

66 ± 1

<1

nd

nd

nd

(S)-Stylopine

nd

100 ± 2b

81 ± 18

nd

nd

nd

(+/−)-Pavine

nd

<1

nd

nd

nd

nd

  1. Values represent the mean ± standard deviation of three independent assays. For each enzyme, activity was calculated relative to the assay showing the highest conversion of substrate (i.e. the average of this assay was set to 100 %). The accompanying footnote defines 100 % conversion in pmole min−1 mg−1 protein for each enzyme
  2. a5.9 pmole min−1 mg−1 protein
  3. b61 pmole min−1 mg−1 protein
  4. c0.4 pmole min−1 mg−1 protein
  5. dProducts were N-methylcoclaurine and N,N-dimethylcoclaurine
  6. e2.3 pmole min−1 mg−1 protein
  7. f 0.1 pmole min−1 mg−1 protein
  8. g3.3 pmole min−1 mg−1