Substrate | Relative activity (% maximum) |
---|
GFLNMT1 | GFLNMT2 | GFLNMT3 | GFLNMT4 | GFLNMT5 | GFLNMT6 |
---|
(S)-Coclaurine | 100 ± 1a
| nd | nd | 100 ± 10d, e
| <1 | 100 ± 14g
|
(S)-Reticuline | nd | nd | 100 ± 5c
| 8 ± 1 | 100 ± 5f
| nd |
(S)-Canadine | nd | 66 ± 1 | <1 | nd | nd | nd |
(S)-Stylopine | nd | 100 ± 2b
| 81 ± 18 | nd | nd | nd |
(+/−)-Pavine | nd | <1 | nd | nd | nd | nd |
- Values represent the mean ± standard deviation of three independent assays. For each enzyme, activity was calculated relative to the assay showing the highest conversion of substrate (i.e. the average of this assay was set to 100 %). The accompanying footnote defines 100 % conversion in pmole min−1 mg−1 protein for each enzyme
-
a5.9 pmole min−1 mg−1 protein
-
b61 pmole min−1 mg−1 protein
-
c0.4 pmole min−1 mg−1 protein
-
dProducts were N-methylcoclaurine and N,N-dimethylcoclaurine
-
e2.3 pmole min−1 mg−1 protein
-
f 0.1 pmole min−1 mg−1 protein
-
g3.3 pmole min−1 mg−1