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Table 3 Relative conversion of five alkaloids tested as potential substrates for six NMT candidates from Glaucium flavum

From: Transcriptome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants

Substrate Relative activity (% maximum)
GFLNMT1 GFLNMT2 GFLNMT3 GFLNMT4 GFLNMT5 GFLNMT6
(S)-Coclaurine 100 ± 1a nd nd 100 ± 10d, e <1 100 ± 14g
(S)-Reticuline nd nd 100 ± 5c 8 ± 1 100 ± 5f nd
(S)-Canadine nd 66 ± 1 <1 nd nd nd
(S)-Stylopine nd 100 ± 2b 81 ± 18 nd nd nd
(+/−)-Pavine nd <1 nd nd nd nd
  1. Values represent the mean ± standard deviation of three independent assays. For each enzyme, activity was calculated relative to the assay showing the highest conversion of substrate (i.e. the average of this assay was set to 100 %). The accompanying footnote defines 100 % conversion in pmole min−1 mg−1 protein for each enzyme
  2. a5.9 pmole min−1 mg−1 protein
  3. b61 pmole min−1 mg−1 protein
  4. c0.4 pmole min−1 mg−1 protein
  5. dProducts were N-methylcoclaurine and N,N-dimethylcoclaurine
  6. e2.3 pmole min−1 mg−1 protein
  7. f 0.1 pmole min−1 mg−1 protein
  8. g3.3 pmole min−1 mg−1