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Fig. 3 | BMC Plant Biology

Fig. 3

From: The Mediator complex subunits MED25/PFT1 and MED8 are required for transcriptional responses to changes in cell wall arabinose composition and glucose treatment in Arabidopsis thaliana

Fig. 3

Comparison of cell elongation and cell wall composition in Col, hsr8-1, pft1-2 and hsr8-1pft1-2. a Hypocotyl cell length was measured from scanning electron micrograph images. n = 10 cells each from 5 hypocotyls. **, p < 0.01 comparing hsr8-1 to Col, and hsr8-1 to pft1-2 and hsr8-1 pft1-2 (Student’s t- test). b Monosaccharide composition of cell wall material isolated from 14 day old light grown seedlings (5 biological replicates). Fuc fucose; Rha rhamnose; Ara arabinose; Xyl xylose; Man mannose. . ***, p < 0.001 comparing arabinose levels in Col to hsr8-1, and pft1-2 to hsr8-1 pft1-2 (Student’s t- test). c Compositional analysis of cell wall material isolated from dark grown hypocotyl tissues using FTIR. The data are represented as differences in relative absorbance from wild-type Col. d Principal Components Analyses of FTIR data. Score loadings of PC1, PC2 and PC3 are plotted against the range of wavelengths to show the major variance

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