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Fig. 4 | BMC Plant Biology

Fig. 4

From: Conservation of tRNA and rRNA 5-methylcytosine in the kingdom Plantae

Fig. 4

Conservation of tRNA methylation in Kingdom Plantae. a Concentric circles from outer to inner represent Arabidopsis thaliana (At), Brassica rapa (Br), Triticum durum (Td), Nannochloropsis oculata (No), Caulerpa taxifolia (Ct) and Ginkgo biloba (Gb) tRNAs, respectively. The circles are split into two major sections for nuclear encoded tRNAs and tRNAs with putative genomic origins (nuclear-N, chloroplast-C, mitochondrial-M). In each circle, individual tRNA consensus sequences are indicated as thick grey arcs and are organized alphabetically by amino acid, and then by anticodon. Specific tRNAs sequences for each species were aligned based on structural positions corresponding to the 72 bp representative tRNA structure. Cytosines that are methylated in at least one of the 6 species analysed are shown as a color-coded percentage methylation bar. The percentage methylation scheme used, Green = lowly methylated (0–40 %), red = highly methylated (80–100 %). Absence of a methylation bar indicates that the corresponding position in the tRNA does not contain a cytosine in that species. A black bar at position 49 in tRNAAla(CGC) in Ct represents an ambiguous nucleotide which may be a C or T at this position. tRNAs that were not detected in the RBS-seq are not shown (Arabidopsis thaliana- 3 biological replicates and all other plant species 1 replicate)

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