Fig. 9

Gene expression in buds after treatment with dsRNA. Quantification of expression levels of the DhMYB1 from untreated buds and buds treated with DhMYB1 dsRNA at stages 5, 6 and 7. Relative expression of DhMYB1 was obtained by dividing the average number of DhMYB1 transcript copies by the copy number of D. hybrida β-actin (endogenous control) for the same tissue. The transcript level of DhMYB1 RNA in stage 7 untreated floral buds was then set to a value of 1 and subsequently expression levels are relative to this number. Bars indicate standard error from triplicate amplification of the DhMYB1 cDNA samples (n = 3). Transcript levels of DhMYB1 gene in treated buds are significantly different from untreated control buds, P < 0.05