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Fig. 6 | BMC Plant Biology

Fig. 6

From: Sucrose accumulation in sweet sorghum stems occurs by apoplasmic phloem unloading and does not involve differential Sucrose transporter expression

Fig. 6

Safranin localization in a stem vein of Wray and Macia at post-anthesis. a-l correspond to Wray tissues, and m-o correspond to Macia tissues. Left, middle, and right columns represent transverse cross-sections of veins shown under bright-field, UV, and green light, respectively. Each row represents a single vein under the different types of illumination. Safranin was first detected in the walls of the xylem elements and adjacent xylem parenchyma cells, as indicated by the red coloration under bright-field (a; arrowhead) and by red fluorescence under green light (c). The safranin also showed some degree of fluorescence under UV illumination (b). Safranin was subsequently present throughout the cell walls of the xylem and was also detectable in the cell walls of the phloem adjacent to the xylem (d-f). Safranin eventually was observed in the cell walls throughout the vein and in the cell walls of the surrounding parenchyma cells (g-i). Control vein not fed safranin (j-l). The same distribution of safranin was observed for a Macia stem vein (m-o). Scale bar = 100 μm

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