Ectopic shoot meristem generation in monocotyledonous rpk1 mutants is linked to SAM loss and altered seedling morphology

Table 2 Frequency of ectopic meristems (eSMs)

Line^a	Dicots & others^b	Monocots + SAM [%]^c	Monocots -SAM^d [%]^c	Monocots + eSM [%]^c	Monocots (+SAM, −SAM, +eSM) [%]^c
rpk1-7 X KNAT2:GUS A	1246	52 [4]	12 [0.9]	0 [0]	4.9
rpk1-7 X KNAT2:GUS B	1902	165 [7.7]	62 [3]	1 [4.5x10⁻⁴]	10.7
rpk1-7 X KNAT2:GUS C	1995	187 [8.3]	62 [2.8]	1 [4.4x10⁻⁴]	11.1
rpk1-7 X KNAT2:GUS D	421	49 [10.1]	12 [2.7]	1 [2.0x10⁻³]	12.8
rpk1-7 X KNAT2:GUS E	332	50 [12.9]	5 [1.5]	1 [2.5x10⁻³]	14.4
rpk1-7 X KNAT2:GUS F	3202	262 [7.4]	78 [2.3]	2 [5.6x10⁻⁴]	9.7

^aOutcrosses to marker line KNAT2p:GUS, repeatedly selfed and with gl1/gl1 and non-KNAT2p:GUS background
^bOnly monocots vs. others were considered, seedlings with irregular cotyledons, e. g. unequally sized (= anisocots), were not separately counted
^cPercentage of all seedlings counted
^dIn three randomly selected batches tested, between 15-66 % of initial –SAM seedlings developed a late SAM

ISSN: 1471-2229