Fig. 6

PRF3 knockout displays elongated petioles, while PRF3 overexpression results in no phenotypic effects. a Petiole length for vegetative PRF T-DNA mutant prf3-2 and its complement lines. b Day 14 primary root length comparison between WT, prf3-2, and three independent A2p:PRF3 overexpression lines. c Day 10 hypocotyl length comparison between WT, prf3-2, and three independent A2p:PRF3 overexpression lines. Seedlings were grown under dark conditions. d Quantification of primary root length measurements from lines pictured in (b). e Quantification of hypocotyl length measurements from lines pictured in (c). f qRT-PCR data on the relative quantity of PRF3 RNA expression for WT, prf3-2, and three independent A2p:PRF3 overexpression lines. g Western blot analysis for WT, prf3-2, and two independent A2p:PRF3 overexpression lines. Western blot bands were quantified using the ImageJ software. All samples were taken from 4w old leaf tissue. Leaf measurements (a) were taken on day 28 (4w) during development, primary root length measurements (d) were taken on day 15, and hypocotyl length measurements (e) were taken on day 10. All measurements’ are in mm. Leaf measurements were generated with a sample of n = 52, while root and hypocotyls measurements have an n = 30. Error bars represent +/- 1 SD. *p value < 0.05