Fig. 2

Analysis of profilin RNA and protein expression for vegetative PRF single T-DNA mutants and complement lines. a The Relative Quantities (RQ) of PRF1 RNA for WT, prf1-4, and A2p:PRF1 plants were determined by quantitative Real Time PCR (qRT-PCR). The RQ of PRF2 RNA for WT, prf2-1, and A2p:PRF2 plants and the RQ of PRF3 RNA for WT, prf3-2, and A2p:PRF3 plants are also shown. Error bars represent +/- 1 SD. b Profilin protein expression was examined by Western blot analysis: WT, prf1-4, and A2p:PRF1 plants using the PRF1 specific monoclonal antibody mAbPRF1a; WT, prf2-1, and A2p:PRF2 plants using the PRF1 and PRF2 specific monoclonal antibody mAbPRF12a; WT, prf3-2, and A2p:PRF3 plants using the PRF1 and PRF2 specific monoclonal antibody mAbPRF12a, which also has weak affinity for PRF3. Coomassie stained gels showing rubisco protein expression are located beneath each blot to show equal loading across lanes. All samples were taken from 4w old leaf tissue