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Table 2 Relative concentration and fold changes of major metabolites in roots of wheat seedlings after 15 d of salt and alkali treatments

From: Comparative metabolic responses and adaptive strategies of wheat (Triticum aestivum) to salt and alkali stress

Metabolic pathways

Metabolites

Relative concentration

Fold changes

CK

SS

AS

log2 (SS/CK)

log2 (AS/CK)

log2 (AS/SS)

TCA cycle

Cit

70.05

114.56

166.40

0.71

1.25a

0.54

Aco

1.37

1.44

2.87

0.07

1.07a

1.00a

α-KG

0.15

0.27

0.59

0.81

1.94a

1.13a

Succ

17.80

11.37

86.02

−0.65

2.27a

2.92a

Fum

1.25

1.04

7.21

−0.27

2.53a

2.80a

Mal

10.44

16.43

18.29

0.65

0.81

0.15

Glycolysis

Glc

22.49

11.19

9.45

−1.01a

−1.25a

−0.24

G6P

0.49

0.23

0.10

−1.09a

−2.32a

−1.23a

F6P

0.19

0.10

0.05

−0.95

−1.96a

−1.01a

3-PGA

0.44

0.27

0.14

−0.72

−1.63a

−0.92

Pyr

0.49

0.52

0.39

0.09

−0.34

−0.43

PEP

0.64

0.61

0.25

−0.07

−1.34a

−1.27a

Amino acids

Glu

134.99

141.95

36.04

0.07

−1.91a

−1.98a

GABA

104.32

46.87

33.20

−1.15a

−1.65a

−0.50

Ala

151.29

98.92

14.73

−0.61

−3.36a

−2.75a

Asp

12.33

7.44

7.28

−0.73

−0.76

−0.03

Gly

7.45

4.09

0.97

−0.87

−2.94a

−2.07a

Thr

9.71

4.95

1.50

−0.97

−2.70a

−1.7a2

Ser

16.05

11.41

2.22

−0.49

−2.85a

−2.36a

Val

17.42

8.19

5.28

−1.09a

−1.72a

−0.64

Pro

8.54

17.36

14.58

1.02a

0.77

−0.25

Phe

0.41

0.19

0.16

−1.08a

−1.31a

−0.23

Gln

0.11

0.12

0.02

0.17

−2.20a

−2.36 a

Ile

7.50

3.06

2.51

−1.29a

−1.58a

−0.29

Leu

0.63

0.19

0.14

−1.73a

−2.18a

−0.44

Asn

16.66

3.07

1.29

−2.44a

−3.69a

−1.25a

Sugars and polyols

Fru

860.33

684.72

237.87

−0.33

−1.85a

−1.53a

Suc

96.20

180.38

104.07

0.91

0.11

−0.79

Tal

119.04

85.27

26.98

−0.48

−2.14a

−1.66a

Kes

60.63

84.66

24.67

0.48

−1.30a

−1.78a

Hep

0.80

0.29

0.18

−1.47a

−2.19a

−0.72

mIno

11.07

24.44

4.62

1.14a

−1.26a

−2.40a

Sor

9.78

3.13

3.42

−1.65a

−1.52a

0.13

Rib

9.02

5.24

1.56

−0.78

−2.53a

−1.75a

Gala

0.85

3.22

0.84

1.92a

−0.02

−1.94a

Raf

0.39

1.01

0.10

1.39a

−1.91a

−3.29a

  1. The relative concentration of each metabolite is an average of data from six biological replicates obtained through GC–MS. Fold changes were calculated using the formula log2 (treatment/control)
  2. aindicates significance (p < 0.05)