Fig. 2

Figure showing the presence of three EF-hands in CBL protein. The green color indicates the 1st EF-hand, red color indicates 2nd EF-hand and orange color indicates 3rd EF-hand. The presence of conserved Asp (D) and Glu (E) amino acids in EF-hands of CBL protein confers binding of calcium ions. Among the three EF-hands, 3rd EF-hand of CBL protein contains E-E-x-D and D-x-D/E calcium binding motifs. All the conserved amino acids (D and E) and motifs present in EF-hands were marked in black color. In the first EF-hand Glu (E) amino acid is conserved at 1, 23 and 24 position and Asp (D) amino acid is conserved at 6, 10, and 13 positions. In second EF-hand Asp/Glu amino acid is conserved at 3, 4 and 7 position but Asp amino acid is significantly conserved at14 position. The Glu amino acid is most significantly conserved at 22 and less significantly conserved at 25 positions. At position 36, Glu amino acid is significantly conserved. In 3rd EF-hand D-D-x-x-E motif is present at 7, 8, 9 10 and 11 position. Asp (D) amino acid is conserved at 15 and 26 position. The E-E-x-D motif is present at 19, 20, and 21 and 22 and D-x-E-E motif is present at 30, 31, 32 and 33 position respectively. The abundance of Asp and Glu amino acids are much more in 3 EF-hand when compared to 1 and 2 EF-hand