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Fig. 9 | BMC Plant Biology

Fig. 9

From: Tomato Sl3-MMP, a member of the Matrix metalloproteinase family, is required for disease resistance against Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000

Fig. 9

Transient expression of Sl3-MMP in N. benthamiana conferred an increased resistance to B. cinerea. a Expression of Sl3-MMP. Agrobacteria carrying pFGC-Sl3-MMP or pFGC-eGFP were infiltrated into leaves of N. benthamiana and expression of Sl3-MMP was analyzed by qRT-PCR. Relative expression levels were calculated by comparing with the corresponding values at 0 h (as a control) after infiltration. b Immunoblot analysis of Sl3-MMP-GFP fusion proteins in N. benthamiana leaves at 48 h after agroinfiltration. A GFP-specific antibody was used for detection of GFP-fusion protein. Equal loading of total proteins was examined by Ponceau staining. c and d Disease symptom and lesion size. e Expression of defense-related genes. Opposite part of the leaves infiltrated with Sl3-MMP-GFP or pFGC-eGFP was inoculated by dropping spore suspension (2 × 105 spores/mL) of B. cinerea and lesion sizes were measured at 5 days after inoculation. Data presented in d are the means ± SD from a minimum of 60 lesions. Data presented in a and e are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level

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