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Fig. 7 | BMC Plant Biology

Fig. 7

From: Tomato Sl3-MMP, a member of the Matrix metalloproteinase family, is required for disease resistance against Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000

Fig. 7

Increased accumulation of H2O2 and cell death in Sl3-MMP-silenced plants after infection with B. cinerea. Two-week-old seedlings were infiltrated with agrobacteria carrying pTRV2-Sl3-MMP or pTRV2-GUS and were inoculated with spore suspension of B. cinerea or with buffer solution as a mock-inoculation control at 4 weeks after VIGS infiltration. Leaves from six individual plants were collected at 24 h after inoculation and used for analysis of cell death and H2O2 accumulation. a and b Accumulation of H2O2 by DAB staining and quantification method respectively. c Cell death detected by trypan blue staining. d Electrolyte leakage. Data presented in b and d are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level

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