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Table 6 Primers used in RT-qPCR for validation of the expression profile obtained by 454 sequencing.

From: Characterization of the cork oak transcriptome dynamics during acorn development

Clustera

Gene abbreviationb

Gene description

Transcript name

At Locus

Primer sequences (forward / reverse)

A

MRP10, ABCC14

ABC transporter C family member 14

Contig3296

AT3G62700

GCTGCCTTTGCCCCACACT/ TGGAAGAGCCTTGAACGCTGCC

A

CPK10, CDPK1

Calcium-dependent protein kinase

Contig4133

AT1G18890

GCTCACCTCCCTCGCACAACT/ AGGCGCGAGGTGGCGATAAT

B

ASPG1

Protein aspartic protease in guard cell

Contig19387

AT3G18490

ATGCAGCTCGCTCGACGTGT/ TGTCGTGTCCGCAACCCAGA

B

ABF2

Abscisic acid responsive elements-binding factor 2

Contig22999

AT1G45249

CGGCCTGCTTTTTCCGCAACT/ AGTCAGCTGCAAGGTCACGAGC

B

AVP1

Pyrophosphate-energized vacuolar membrane proton pump

Contig 8793

AT1G15690

CGCACTTGAGAACGACGCT/ TGCGCGGTCGTCGGAATCAT

B

HSP90-7

Chaperone protein htpG family protein

Qs-dev_rep_c72408

AT4G24190

CAGCATCAGCTTCAGCCTCGGT/ AGCGGCTTCACGCCTTCTGA

B

BAM1, BMY7, TR-BAMY

β-amylase 1

Contig 25491

AT3G23920

AACGCGAGTTTGCAGGCGCT/ CGACGTTTCCGCCGCATTGA

B

GolS2

Galactinol synthase 2

Contig 17943

AT1G56600

TGACGCCATAACATGGCCAGCA/ TTGCCAGCAGTGCCCTGACA

C

ALDH7B4

Aldehyde dehydrogenase 7B4

Contig 9124

AT1G54100

AGGGTGCTCCAACGACTCCA/ ACGCACAGCTAGGCCGATG

C

OPR3

Oxophytodienoate-reductase 3

Contig 16616

AT2G06050

TTGGTCTCTAACACAGCCGCCG/ CACGGCCGACATGCCATA

C

PAL1

Phenylalanine ammonia-lyase 1

Contig 9321

AT2G37040

TGCTAACTGGCCGCCCCAAT/ GCCAGAACCAACAGCTGTGCCA

D

ABI3, SIS10

Abscisic acid insensitive 3

Contig 19318

AT3G24650

GCAGTGGCCGTGGTGCAAT/ ATGGCGAGGCAAAGGCGGTT

D

PHS1

Glycosyl transferase, family 35

Contig 10143

AT3G29320

TGGCTTGAGATGGGCAACCCT/ TTTGGTGTGCTCCCCGGCAT

D

SIR

Sulfite reductase

Contig 19883

AT5G04590

TGCAATGGCATGCCCAGCCT/ ACTGGGGTTTCCACCAAGCCA

D

PIP2B

Aquaporin PIP2-4

Contig 24062

AT2G37170

GCTTGGGCCTTTGGTGGCA/ TGGCTCCACCACCGTACTTGCT

F

COR414-TM1

Cold-regulated 314 inner membrane 1

Contig 19349

AT1G29395

TGGTGAGCTTGAGCTGCCGT/ TGCAAGCTGCCTGCTCGTGA

F

DI21

Late embryogenesis abundant protein

Contig 16865

AT4G15910

AGCGTGCTCTCACCTTGTGGT/ TGCGGCTGCATCACATGCCT

F

JMT

Jasmonic acid carboxyl methyltransf.

Contig 17776

AT1G19640

TGGCTATCTTCGCCGCCCTT/ TGCCGTGGTTTGCACCTACTTCG

F

GSTU25

Glutathione S-transferase TAU 25

Contig 17981

AT1G17180

ACCACCTCGTCCGCCATTGT/ AGCCTGACCGACTCCATGGCAA

F

LTP3

Lipid transfer protein 3

Contig 19993

AT5G59320

TGCCCAGGCCACCATAACATGC/ TGTGGTCTTGGCCATGCCGTT

  1. a According to their expression profiles each transcript belongs to a different cluster
  2. b Corresponds to the homologous in Arabidopsis thaliana