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Fig. 8 | BMC Plant Biology

Fig. 8

From: OsGRAS23, a rice GRAS transcription factor gene, is involved in drought stress response through regulating expression of stress-responsive genes

Fig. 8

Identification of putative target genes regulated by OsGRAS23 with yeast one hybrid assay. a Schematic structure of yeast expression construct pGAD-OsGRAS23 and reporter construct pHIS2.1-GTP (OsGRAS23 putative target gene promoter). b Growth performance of transformants on SD/-Leu-/Trp/-His medium containing 100 mM or 30 mM 3-AT. GTP1-GTP8 indicates the pGAD-OsGRAS23 plus pHIS2.1-cis (promoters of Os07g0162450, Os01g0537250, Os07g0638400, Os03g0629800, Os04g0173800, Os03g0289800, Os06g0513781, and Os07g0673900 in pHIS2.1, respectively). ck-: negative control (pGADT7-rec2-OsGRAS23 plus p53HIS2.1)

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