Table 1 Sequences of the primers used in the gene expression study
Gene | Protein | Locus ID | Primer | Sequence (5’ to 3’) |
|---|---|---|---|---|
ANS (LDOX) | leucoanthocyanidin dioxygenase | Tc03g026420 | TcANS_RTF | ACCTTGTTAACCATGGGATCTCGG |
TcANS_RTR | GACGGTGTCACCAATGTGCATGAT | |||
ANR | anthocyanidin reductase | Tc06g018030 | TcANR_RTF | TGCTTGAGAAGGGCTACGCTGTTA |
TcANR_RTR | AAAGATGTGGCAAGGCCAATGCTG | |||
LAR | Leucoanthocyanidin reductase | Tc03g002450 | TcLAR_RTF | AATTCCATTGCAGCTTGGCCCTAC |
TcLAR_RTR | GGCTTGCTCACTGCTTTGGCATTA | |||
UB | polyubiquitin | Tc06g018641 | TcUb_RTF | AGCTGAGAGATTCCGTTGTCCAGA |
TcUb_RTR | CCCACATCAACCAGACTTTGAGTTC | |||
MybPA | TT2 like MYB transcriptoin factor | Tc01g034240 | Tc-MYBPA_RTF | GATGGGAAGGGCTCCTTGTTG |
Tc-MYBPA_RTR | ATCTCGTTATCGGTTGGACCAG | |||
DFR | dihydroflavonol 4-reductase | AT5G42800 | AtDFR_RTF | CCGTGTGTGTAACCGGCGCT |
AtDFR_RTR | TGCGCCTCGTTCCGAGTGAT | |||
LDOX (ANS) | leucoanthocyanidin dioxygenase | AT4G22880 | AtLDOX_RTF | CACCAAGTGATTACATAGAAGCAACG |
AtLDOX_RTR | TCACCATCTCCGGCAACGGC | |||
BAN (ANR) | anthocyanidin reductase | AT1G61720 | AtBAN_RTF | TGGTGGCACGGGAAACTTAGCC |
AtBAN_RTR | CGGTCACATGCATTTCTTTCCCGGT | |||
UFGT | UDP-glucosyl transferase | AT5G17050 | AtUFGT_RTF | ACCATCGGTGTCAAAGAAGTAGGTG |
AtUFGT_RTR | GCGGTGCCCATGGAACCACT | |||
CHS | chalcone synthase | AT5G13930 | AtCHS_RTF | TCAAGCGCATGTGCGACAAGT |
AtCHS_RTR | CGGCGGCGCCATCACTGAAA | |||
CHI | chalcone isomerase | AT3G55120 | AtCHI_RTF | TCATCCAACGCCTGCGCCTC |
AtCHI_RTR | ACGCAACCGTAAGAGAGCCGGT | |||
F3H | flavanone 3-hydroxylase | AT3G51240 | AtF3H_RTF | ACCTCCAGGGAGAGGCTGTGC |
AtF3H_RTR | AAATGGCCGTGGTCGCCGAG | |||
FLS | flavonol synthase 1 | AT5G08640 | AtFLS_RTF | ATCTGGCCACCGTCATGCGTC |
AtFLS_RTR | CCTCCCATTACTCAACCTCAGAATC |