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Fig. 5 | BMC Plant Biology

Fig. 5

From: The maize cytochrome P450 CYP79A61 produces phenylacetaldoxime and indole-3-acetaldoxime in heterologous systems and might contribute to plant defense and auxin formation

Fig. 5

Aldoxime accumulation a and volatile emission b of transgenic N. benthamiana plants overexpressing maize CYP79A61. Plants were infiltrated with A. tumefaciens containing 35S:eGFP (control) or 35S:CYP79A61. Aldoximes were extracted three days after infiltration with methanol and analyzed using LC-MS/MS. Volatiles were collected on the third day after infiltration. Identification of volatile compounds was done with GC-MS and quantification was done with GC-FID. PAld, 2-phenylacetaldehyde; 2PE, 2-phenylethanol; BC, benzyl cyanide; PN, 2-phenylnitroethane; (E)-PAOx, (E)-phenylacetaldoxime; (Z)-PAOx, (Z)-phenylacetaldoxime. Means and standard errors are shown (n = 5)

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