Skip to main content
Fig. 4 | BMC Plant Biology

Fig. 4

From: The UDP-glucose: glycoprotein glucosyltransferase (UGGT), a key enzyme in ER quality control, plays a significant role in plant growth as well as biotic and abiotic stress in Arabidopsis thaliana

Fig. 4

The atuggt1-1 and atuggt1-2 mutants exhibit a reduced uptake of UDP-[14C]Glc into ER-derived vesicles. The UDP-Glc uptake was assayed into 50 μg of ER-enriched fractions from wild-type and AtUGGT mutant plants incubated with 1 μM UDP-[14C]Glc for 15 min. The reaction was stopped with a 10-fold dilution with cold STM buffer and filtration. The filter-associated label was counted using liquid scintillation. Results are presented as mean with SD; significance was determined with ANOVA. Asterisks indicate a Tukey’s test p-value < 0.001

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us