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Fig. 3 | BMC Plant Biology

Fig. 3

From: The UDP-glucose: glycoprotein glucosyltransferase (UGGT), a key enzyme in ER quality control, plays a significant role in plant growth as well as biotic and abiotic stress in Arabidopsis thaliana

Fig. 3

UGGT is induced during UPR and UGGT mutants that exhibit ER stress. a Quantitative real-time PCR monitoring of AtUTr1, BiP1/2, BiP3, PDIL2-1 and AtUGGT transcript levels in stressed wild type plants. Fifteen day-old seedlings were treated over 5 hrs with DTT 2.5 mM or TUN 5 μg/ml in MS medium. Clathrin adapter (At5g46630) was used as a housekeeping gene. The average values of three independent experiments (n = 6) are shown; error bars represent ± SD. b Quantitative real-time PCR monitoring AtUTr1, BiP1/2, BiP3, PDIL2-1 and AtUGGT transcript levels in wild type and AtUGGT mutant plants grown under normal conditions. Fifteen day-old seedlings were used for the analysis. Clathrin adapter (At5g46630) was the housekeeping gene. The average values of three independent experiments (n = 6) are shown; error bars represent ± SD

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BMC Plant Biology

ISSN: 1471-2229

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