Figure 2

Comparative alignment and phylogenetic tree of lycopene β-cyclase. (A) The alignment was created using ClustalW. The amino acid residues which are identical in all sequences are shown in white text on a black background, whereas different residues are shown in black text on a white background. Characteristic regions of plant LCYBs are indicated under the LCYB sequence: Conserved LCYB region, Di-nucleotide binding site, LCY’s specific motif, Cyclase motifs (CM) I and II, Predicted TM helices, Charged region and β-LCY CAD (Catalytic Activity Domain). AtLCYB: A. thaliana lycopene β-cyclase; CaLCYB: C. annuum lycopene β-cyclase; SlLCYB: S. lycopersicum lycopene β-cyclase; ZmLCYB: Z. mays lycopene β-cyclase; OsLCYB: O. sativa lycopene β-cyclase. (B) The multiple alignments were generated by ClustalW and the phylogenetic tree was constructed with MEGA4.0 using a bootstrap test of phylogeny with minimum evolution test and default parameters.