Fig. 4

Relative gene expression levels of CaHsfs, analysed by qRT-PCR, in response to HS treatment in B6 and R9 leaves. HS treatment: 40 °C for 2 h; B6: pepper thermosensitive line; R9: pepper thermotolerant line. qRT-PCR data were normalized using the pepper ubiquitin-conjugating protein gene (UBI-3) and are shown relative to 0 h. The relative expression levels were calculated using the -ΔΔCT method and then a heat map with HemI was created