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Table 2 Substrate specificity of GmF3G2″Gt in cultivar Nezumisaya

From: Linkage mapping, molecular cloning and functional analysis of soybean gene Fg3 encoding flavonol 3-O-glucoside/galactoside (1 → 2) glucosyltransferase

 

Relative activity (%)

Sugar acceptor a

 

Kaempferol (Kae) 3-O-glucosideb

100.0 ± 5.3

Kae 3-O-galactoside

73.7 ± 0.5

Kae 3-O-rhamnosyl-(1 → 6)-glucoside

42.4 ± 3.7

Kae 3-O-rhamnosyl-(1 → 4)-[rhamnosyl-(1 → 6)-glucoside]

N.D.

Kae 3-O-rhamnosyl-(1 → 4)-[glucosyl-(1 → 6)-glucoside]

N.D.

Kae 3-O-glucoside-7-O-rhamnoside

23.8 ± 1.1

Quercetin (Que) 3-O-glucoside

75.2 ± 4.9

Que 3-O-galactoside

78.9 ± 2.4

Que 3-O-rhamnosyl-(1 → 6)-glucoside

25.4 ± 1.3

Sugar donor c

 

UDP-glucose

100.0 ± 5.2

UDP-galactose

3.3 ± 0.1

UDP-arabinose

N.D.

UDP-glucuronic acid

N.D.

  1. N.D., not detected.
  2. aThe reactions were performed with UDP-glucose as the sugar donor.
  3. bThe product was identified based on comparison with the pertinent standard.
  4. cThe reactions were performed with kaempferol 3-O-glucoside.
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BMC Plant Biology

ISSN: 1471-2229

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