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Table 2 Substrate specificity of GmF3G2″Gt in cultivar Nezumisaya

From: Linkage mapping, molecular cloning and functional analysis of soybean gene Fg3 encoding flavonol 3-O-glucoside/galactoside (1 → 2) glucosyltransferase

  Relative activity (%)
Sugar acceptor a  
Kaempferol (Kae) 3-O-glucosideb 100.0 ± 5.3
Kae 3-O-galactoside 73.7 ± 0.5
Kae 3-O-rhamnosyl-(1 → 6)-glucoside 42.4 ± 3.7
Kae 3-O-rhamnosyl-(1 → 4)-[rhamnosyl-(1 → 6)-glucoside] N.D.
Kae 3-O-rhamnosyl-(1 → 4)-[glucosyl-(1 → 6)-glucoside] N.D.
Kae 3-O-glucoside-7-O-rhamnoside 23.8 ± 1.1
Quercetin (Que) 3-O-glucoside 75.2 ± 4.9
Que 3-O-galactoside 78.9 ± 2.4
Que 3-O-rhamnosyl-(1 → 6)-glucoside 25.4 ± 1.3
Sugar donor c  
UDP-glucose 100.0 ± 5.2
UDP-galactose 3.3 ± 0.1
UDP-arabinose N.D.
UDP-glucuronic acid N.D.
  1. N.D., not detected.
  2. aThe reactions were performed with UDP-glucose as the sugar donor.
  3. bThe product was identified based on comparison with the pertinent standard.
  4. cThe reactions were performed with kaempferol 3-O-glucoside.
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BMC Plant Biology

ISSN: 1471-2229

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