Outline and results of dCAPS analysis of GmF3G2″Gt in soybean. (A) Schematic presentation of dCAPS analysis. Partial nucleotide sequences around the region polymorphic between Harosoy and Nezumisaya are exhibited. A mismatched base in the forward primer is highlighted in gray. Polymorphic nucleotides are highlighted in black. (B) Results of dCAPS analysis of the parents and the recombinant inbred lines derived from a cross between Nezumisaya and Harosoy. PCR products amplified with dCAPS primers were digested by KpnI and the digests were separated on an 8% polyacrylamide gel. ϕ, molecular marker ϕx174/HaeIII; N, Nezumisaya; H, Harosoy. FG pattern of the recombinant inbred lines is exhibited below the gel. H, Harosoy-type; N, Nezumisaya-type, 3, type 3; 4, type 4. The migration of size markers (bp) is shown to the left of the gel.