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Figure 4 | BMC Plant Biology

Figure 4

From: AKIN10 delays flowering by inactivating IDD8 transcription factor through protein phosphorylation in Arabidopsis

Figure 4

Identification of phosphorylation residues in IDD8. A Predicted phosphorylation residues in IDD8. Potential phosphorylation residues were predicted using the NetPhos-based analysis tool (http://www.cbs.dtu.dk/services/NetPhos/). The predicted serine (S) and threonine (T) residues were mutated to alanine (A). ZF, zinc finger. aa, amino acid. B in vitro phosphorylation assay. The assays were conducted using recombinant GST-AKIN10 and MBP-IDD8 fusion proteins prepared in E. coli cells (upper panel). Part of Coomassie Blue-stained gel was displayed as a loading control (middle panel). Black arrowheads indicate IDD8 protein. White arrowheads indicate AKIN10 protein. kDa, kilodalton. The relative intensities of the phosphorylation bands were calculated in comparison to those on Coomassie Blue-stained gel (lower panel). Experimental triplicates were averaged and statistically analyzed using Student t-test (*P < 0.01, **P < 0.05, difference from wild-type IDD8). Bars indicate standard error of the mean.

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