IDD8 interacts with AKIN proteins in the nucleus. A
in vitro pull-down assay. Recombinant GST-AKIN10 and GST-AKIN11 fusion proteins produced in E. coli cells and in vitro translated, radio-labelled IDD8 polypeptides were used (upper panel). Recombinant GST was used as negative control. The ‘Input’ represents 20% of the labeling reaction. Part of Coomassie Blue-stained gel was displayed as a loading control (lower panel). kDa, kilodalton. B BiFC assay. nYFP-IDD8 and cYFP-AKIN fusions and cyan fluorescent protein (CFP)-ICE1 fusion, which was used as a nuclear marker, were coexpressed transiently in Arabidopsis protoplasts. IDD8-AKIN interactions were visualized by differential interference contrast (DIC) and fluorescence microscopy. Scale bars, 10 μm.