Figure 5

Heat map of transcript profile of defense-related genes in P. emaculata inoculated Bd21 leaves. Transcript profiling of defense-related genes in Bd21 leaf tissue collected at 0, 12, 24, 48 and 72 hours after inoculation (hai) with P. emaculata or after treatment with water containing 0.05% Tween20 (mock). For each treatment, leaves of at least five plants were pooled for each replication with a total of three replications per treatment. Relative quantification (in fold change) of P. emaculata and mock treated samples was calculated in relation to 0 hai and given a value of one. For each time-point, change in gene expression was calculated by measuring ratios of fold change between P. emaculata inoculated and mock inoculated samples. Intensity of red and green color indicates the extent of upregulation and downregulation, respectively, with respect to the gene expression at 0 hai which has been normalized to 1 (yellow). Numerical values of fold change are given in parenthesis in each box. 0, 12, 24, 48 and 72 represent hai. Three technical replicates were used for each sample.