Genomic organization and alternative splicing of OsTCTP. (A) RT-PCR analysis using a primer pair covering the differentiated regions of the OsTCTPa and OsTCTPb cDNAs. The root and shoot mRNAs from cv. ‘Nipponbare’ were used for RT-PCR. (B) Southern blot analysis of the OsTCTP gene. Total DNA from cv. ‘Nipponbare’ (30 μg for each lane) was digested individually with EcoRI, HindIII and XhoI and hybridized with a gene-specific probe covering the region from nucleotide 513 to the 3’ end of the OsTCTPa cDNA. (C) Genomic PCR analysis using two primers covering the differentiated regions of the OsTCTPa and OsTCTPb. The leaf DNA from cv. ‘Nipponbare’ was used for genomic PCR. M, DNA size markers.