Table 1 Details of salt tolerance gene, respective genbank LOC number, motifs with repeat number and location in sequence, primers with Tm and molecular weight of expected band

LOC_Os11g08210

OsNac5

ATGTGATTAGAGTCGCTTTCAGTTGG (56.9C)

CCAGCTTGTACTTGTGCCAGCC (58.4C)

238 bp

10

0.110

TF

(TAA)₁₈

3′UTR

LOC_Os10g25010

OsCML8

GAGAATCAGAGCAAGAGTCTGAACCAGC (61C)

GTCAGCCGCTTCTTCCTCACCTG (61C)

209 bp

21

0.275

Signaling

(CGG)₉

CDS

LOC_Os01g32120

OsCML11

CATGCAAGCCTGCGGAGACG (61.2C)

CGGTCGAAGGAGCGGAAGATCT (60.5C)

154 bp

42

0.221

Signaling

(CAG)₁₀

CDS

LOC_Os02g17500

OsGMST1

AGGAACCAACAGAAGCAAAGGTG (56.3C)

GAGGTGATTTGATGCTGTGAGGC (57.4C)

194 bp

25

0.289

Sugar Transporter

(AG)₁₀

5′UTR

LOC_Os07g06740.2

OsCPK17

TTGCCTTTTGATCTAGTGCATTGG (57.2C)

GTCTTCGTCCTTTACTAAATAGCACTCC (55.8C)

267 bp

21

0.353

Kinase

(CT)₉/(CT)₁₁

3′UTR

LOC_Os02g04630

OsCAX (D)

CTGTTTGGCAATCTGCCAGC (55.6C)

CGTCTCGGCAAAATGTTCCTC (56.1C)

139 bp

20

0.326

Ion transporter

(CT)₁₈

Intronic

LOC_Os02g04630

OsCAX (T)

CTTTGGTTGGTTCAGGACGATG (55.9)

GAATTGGAAGCTGTTGGCTCATTC (57.9)

163 bp

18

0.169

“

(TTA)₂₆

Intronic

LOC_Os07g38090

OsC3H50

GAGGAATTAGACCATTTAACTCGTCGC (58.7)

GAATCCGACCCAATCCAATCAAG (58.3)

214 bp

24

0.199

RNA processing

(TC)₉

5′UTR

LOC_Os06g48590.1

OsMAPK4

GACATCTAAGTGCCGCGTGTTC (56.2)

TACATGCAGCGTCGAATCGAAG (57.6)

254 bp

17

0.358

Kinase

(CT)₁₂

5′UTR

LOC_Os01g54600

OsWRKY13

CCATGCGTACATACACGTTCATGTG (57C)

GATGGGTGCAGCTTTCAATGATC (57.3C)

246 bp

20

0.370

TF

(AG)₁₆/(GA)₉

5′UTR

LOC_Os01g72530.1

OsCML31

GTTGATGGATCTGTAAATGCTTCATGG (58.8)

GGCACCATGGAGCACCAAAC (57.4)

167 bp

Not amplified

Not amplified

Signaling

(AT)₄₀

3′UTR

LOC_Os01g45274.1

OsCA1

CCATCGAGTACGCCGTCTGC (57.9)

CTTCACCATGAATGTTACACACCCTAC (56.8)

281 bp

35

0.296

Chloroplast photosynthesis

(CT)₉

Intronic

LOC_Os02g02840.1

OsRacB (D)

GCTCCTCCTTCAACCTTCTTCTTTC (57.1C)

GTGACGCACTTTATGAACCTGGAC (56.5C)

176 bp

30

0.318

Signaling, GTPase

(GA)₂₁

5′UTR

LOC_Os02g02840.1

OsRacB (T)

CAAGACCTGCATGCTCATCTCC (56.1C)

CCAGATCAAGAACCATAATCCTAGCTC (56.9C)

202 bp

14

0.386

“

(TTC)₉

Intronic

LOC_Os05g51670.1

OsUGE1

CACAACGCCAACAACCTCGAC (57.7C)

GCTTATCGAGATGGGAATGGTTG (56.5)

154 bp

11

0.087

Nucleotide sugar metabolism

(TC)₉

Intronic

LOC_Os06g48590.1

OsMSRMK3

CACCTCCATTTCCCATTCCACC (58.9C)

CGAATCGAAGGCGGCAGCTATAG (60.9)

201 bp

27

0.340

Signaling, Kinase

(CT)₁₂

5′UTR

LOC_Os02g35190.2

OsCLC-1

CAGAGAAGCCAAGCAAAGAAAGTCTC (58.1C)

CCGTGCTCTCGATGTCGTAGTTG (59.2)

179 bp

24

0.322

Ion trasport

(AGA)₁₁

5′UTR

LOC_Os09g13570

OsbZIP71

CTCAGTAAGCTCCCTGTAGTTGTAGCC (57.3)

GTTCAGGTCATCTTCCGACCTGG (58.5)

259 bp

13

0.323

TF

(TA)₁₂

5′UTR

LOC_Os03g02590

OsPEX11-1

GCTGCTCTCGACTTTCTTGTTCC (56.2)

ACTAGCCCTGCACAGACTGAAGAG (55.8)

276 bp

21

0.261

Peroxisomal biogenesis

(TG)₁₉

Intronic