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Figure 5 | BMC Plant Biology

Figure 5

From: Mineral nitrogen sources differently affect root glutamine synthetase isoforms and amino acid balance among organs in maize

Figure 5

Analysis of phosphorylation state of root GS isoforms. The 2D-electrophoretic profiles in 4–7 pI range of soluble proteins from root of maize plants grown in the different experimental conditions were stained with sequential fluorescence staining procedures. A. Representative magnification of the profile containing the GS isoforms stained with Sypro Ruby®, showing all the proteins. B. Representative magnification of the same gel portion after the Pro-Q® Diamond phosphoprotein gel staining that points out only the phosphorylated proteins. The experiment was conducted for each nutritional condition showing a similar profile for all the samples. The spots are numbered (1–6) according to Figure 3 and Table 1. The molecular masses of electrophoretic standards are reported on the left (kDa).

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