Figure 3

Localization of the GS isoforms in 2D profile of maize roots by Western Blotting. Representative 2D-Electrophoretic map of soluble protein fraction from roots of maize plants. Proteins were analyzed by isoelectric focusing at pH 4–7 and 10% SDS-PAGE and visualized by cCBB staining (A). For each nutritional treatments, the gel portion comprised in the broken-line was analyzed by WB against all GS (B, GS1 + GS2 global antibody). The analyses were conducted twice on two independent biological samples (n = 4) showing the same profile among all the experimental conditions. The visualized spots are numbered (1–6) and traced on the 2D-Electrophoretic map. The LC-nESI-MS/MS characterizations of the six spots are reported in Table 1. The molecular masses of electrophoretic standards are reported on the left (kDa).