Arabidopsis overexpressing TcBBM leads to spontaneous regeneration from the cotyledon A, D. E12-Ω-CaMV-35S::TcBBM (BBM-N) Arabidopsis line showing spontaneous regeneration of cotyledon like structures from the seedling cotyledons (black arrows). B. E12-Ω-CaMV-35S::TcBBM (BBM-CD) Arabidopsis line showing no phenotype. C. Arabidopsis Col 0 wild type. E. The corresponding TcBBM levels of the three E12-Ω-CaMV-35S::TcBBM lines shown in images A, B and C. Expression levels were analyzed by RT-qPCR and the TcBBM gene normalized relative to AtPP2a and AtUBQ10. Image scale bars = 1 mm.