Figure 4

Relationships between the expression levels of pAMT and Pun1 genes, and the accumulation of capsaicin in different pepper cultivars. (A) pAMT expression in different pepper cultivars. RNA transcript levels for the pAMT gene at 25 days after flowering in placental tissues were determined using quantitative RT-PCR (mean ± SE; n = 3). ‘Chosen’ and ‘Gekikara’: pungent pepper cultivars; ‘Nikko’ and ‘Fukumimi’: mildly pungent pepper cultivars; ‘Fushimi’ and ‘Manganji’: nonpungent pepper cultivars. (B) Pun1 gene expression in different pepper cultivars. Pun1 transcript levels were determined using quantitative RT-PCR (means ± SE; n = 3) with the same RNA as that used for the pAMT analyses. (C) Capsaicin levels in pungent and nonpungent pepper cultivars as determined by HPLC.