Figure 1

Western blot analysis of the Pun1 protein in the pungent variety ‘Chosen’ and the nonpungent bell pepper using anti-Pun1 antibodies. As indicated by the arrow, the Pun1 protein was detected in the total proteins isolated from placental tissues of ‘Chosen’ by polyclonal antibodies against the E. coli-synthesized Pun1 protein (left). The Coomassie brilliant blue-stained gel is shown as a loading control (right). Asterisks show nonspecific bands.