PCR marker | Name | Primer (5′→3′) | Target allele(s) | Expected product size (bp) | Annealing temp. (ºC) | Reference. |
---|---|---|---|---|---|---|
VRN-A1 marker* | Vrn1AF | GAAAGGAAAAATTCTGCTCG | VRN-A1a | 876 and 965 | 55.0 | |
 | Int1R | GCAGGAAATCGAAATCGAAG | VRN-A1b | 694 |  |  |
 |  |  | VRN-A1d | 662 |  |  |
 |  |  | VRN-A1h | 684 |  |  |
 |  |  | VRN-A1f | 703 |  |  |
 |  |  | VRN-A1 | 704 |  |  |
 |  |  | VRN-A1u | 705 |  |  |
 |  |  | VRN-A1u | 713 |  |  |
T. monococcum VRN-A1 | Indel(-)F | CGCTCTTATATTTGTTTACCAGGG | VRN-A1 | 1025 | 50.0 | _ |
Indel(-)R | GGGTCAACTATTCTGTGGAG | VRN-A1u, VRN-A1u’ | no product |  |  | |
VRN-A1 deletion of 1.4 kb | Intr1/C/F | GCACTCCTAACCCACTAACC | VRN-A1u, VRN-A1u’ | 1068 | 56.0 | [24] |
Intr1/AB/R | TCATCCATCATCAAGGCAAA | Â | Â | Â | Â | |
VRN-A1 insertion of 0.5 kb | Intr 1 | ATCATCTTCTCCACCAAGGG | VRN-A1ins | 1980 | 50.0 | _ |
Intr1insR | AATGAACAGCACGGAAACAG | VRN-A1 | 1476 | Â | Â | |
VRN-A1 deletion of 7,2 kb | Ex1/C/F | GTTCTCCACCGAGTCATGGT | VRN-A1L | 522 | 55.6 | [24] |
Intr1/A/R3 | AAGTAAGACAACACGAATGTGAGA | Â | Â | Â | Â | |
VRN-B1 marker* | P2 | TCATGCACGCACACACGGTA | VRN-B1 | 814 | 55.0 | [25] |
P5 | GGCCAACCCTACACCCCAAG | Â | Â | Â | Â | |
VRN-B1 Non-deletion | Intr1/B/F | CAAGTGGAACGGTTAGGACA | VRN-B1 | 1149 | 56.4 | [24] |
Intr1/B/R4 | CAAATGAAAAGGAATGAGAGCA | Â | Â | Â | Â |