PCR marker
|
Name
|
Primer (5′→3′)
|
Target allele(s)
|
Expected product size (bp)
|
Annealing temp. (ºC)
|
Reference.
|
---|
VRN-A1 marker*
|
Vrn1AF
|
GAAAGGAAAAATTCTGCTCG
|
VRN-A1a
|
876 and 965
|
55.0
|
[16,23,31]
|
|
Int1R
|
GCAGGAAATCGAAATCGAAG
|
VRN-A1b
|
694
| | |
| | |
VRN-A1d
|
662
| | |
| | |
VRN-A1h
|
684
| | |
| | |
VRN-A1f
|
703
| | |
| | |
VRN-A1
|
704
| | |
| | |
VRN-A1u
|
705
| | |
| | |
VRN-A1u
|
713
| | |
T. monococcum VRN-A1
|
Indel(-)F
|
CGCTCTTATATTTGTTTACCAGGG
|
VRN-A1
|
1025
|
50.0
|
_
|
Indel(-)R
|
GGGTCAACTATTCTGTGGAG
|
VRN-A1u, VRN-A1u’
|
no product
| | |
VRN-A1 deletion of 1.4 kb
|
Intr1/C/F
|
GCACTCCTAACCCACTAACC
|
VRN-A1u, VRN-A1u’
|
1068
|
56.0
|
[24]
|
Intr1/AB/R
|
TCATCCATCATCAAGGCAAA
| | | | |
VRN-A1 insertion of 0.5 kb
|
Intr 1
|
ATCATCTTCTCCACCAAGGG
|
VRN-A1ins
|
1980
|
50.0
|
_
|
Intr1insR
|
AATGAACAGCACGGAAACAG
|
VRN-A1
|
1476
| | |
VRN-A1 deletion of 7,2 kb
|
Ex1/C/F
|
GTTCTCCACCGAGTCATGGT
|
VRN-A1L
|
522
|
55.6
|
[24]
|
Intr1/A/R3
|
AAGTAAGACAACACGAATGTGAGA
| | | | |
VRN-B1 marker*
|
P2
|
TCATGCACGCACACACGGTA
|
VRN-B1
|
814
|
55.0
|
[25]
|
P5
|
GGCCAACCCTACACCCCAAG
| | | | |
VRN-B1 Non-deletion
|
Intr1/B/F
|
CAAGTGGAACGGTTAGGACA
|
VRN-B1
|
1149
|
56.4
|
[24]
|
Intr1/B/R4
|
CAAATGAAAAGGAATGAGAGCA
| | | | |
- *These diagnostic markers detect allelic variation at the promoter regions. In other cases variation within intron 1 of corresponding genes is detected.