VRN-1 gene- associated prerequisites of spring growth habit in wild tetraploid wheat T. dicoccoides and the diploid A genome species

Table 1 PCR markers for determining the presence of different alleles of VRN-A1 , VRN-B1 in diploid and polyploid wheats

PCR marker	Name	Primer (5′→3′)	Target allele(s)	Expected product size (bp)	Annealing temp. (ºC)	Reference.
VRN-A1 marker*	Vrn1AF	GAAAGGAAAAATTCTGCTCG	VRN-A1a	876 and 965	55.0	[16,23,31]
	Int1R	GCAGGAAATCGAAATCGAAG	VRN-A1b	694
			VRN-A1d	662
			VRN-A1h	684
			VRN-A1f	703
			VRN-A1	704
			VRN-A1u	705
			VRN-A1u	713
T. monococcum VRN-A1	Indel(-)F	CGCTCTTATATTTGTTTACCAGGG	VRN-A1	1025	50.0	_
T. monococcum VRN-A1	Indel(-)R	GGGTCAACTATTCTGTGGAG	VRN-A1u, VRN-A1u’	no product
VRN-A1 deletion of 1.4 kb	Intr1/C/F	GCACTCCTAACCCACTAACC	VRN-A1u, VRN-A1u’	1068	56.0	[24]
VRN-A1 deletion of 1.4 kb	Intr1/AB/R	TCATCCATCATCAAGGCAAA
VRN-A1 insertion of 0.5 kb	Intr 1	ATCATCTTCTCCACCAAGGG	VRN-A1ins	1980	50.0	_
VRN-A1 insertion of 0.5 kb	Intr1insR	AATGAACAGCACGGAAACAG	VRN-A1	1476
VRN-A1 deletion of 7,2 kb	Ex1/C/F	GTTCTCCACCGAGTCATGGT	VRN-A1L	522	55.6	[24]
VRN-A1 deletion of 7,2 kb	Intr1/A/R3	AAGTAAGACAACACGAATGTGAGA
VRN-B1 marker*	P2	TCATGCACGCACACACGGTA	VRN-B1	814	55.0	[25]
VRN-B1 marker*	P5	GGCCAACCCTACACCCCAAG
VRN-B1 Non-deletion	Intr1/B/F	CAAGTGGAACGGTTAGGACA	VRN-B1	1149	56.4	[24]
VRN-B1 Non-deletion	Intr1/B/R4	CAAATGAAAAGGAATGAGAGCA

*These diagnostic markers detect allelic variation at the promoter regions. In other cases variation within intron 1 of corresponding genes is detected.

ISSN: 1471-2229