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Figure 3 | BMC Plant Biology

Figure 3

From: Wheat homologs of yeast ATG6 function in autophagy and are implicated in powdery mildew immunity

Figure 3

Autophagy-related ATG6 genes function in wheat autophagy. (A) Functional complementation of atg6-mutant yeast cells by wheat ATG6s. The autophagic bodies accumulated within vacuoles are indicated by arrows. Scale bars represent 5 μm. (B) Subcellular localization of GFP-TaATG6 fusion structures in onion epidermal cells. Scale bars represent 50 μm. (C) Localization of GFP-TaATG6a in cytoplasmic and perinuclear membrane-like structures in onion epidermal cells. Florescence spots localized in cytoplasmic and perinuclear membrane-like structures are indicated by arrows. Scale bars represent 10 μm. (D) Relative transcript levels of wheat ATG6s in leaves of barley stripe mosaic virus (BSMV)-treated plants. The fourth leaves were sampled from BSMV-treated 92R137/Yangmai 1587 (Pm21) plants under normal conditions and used in the quantification of TaATG6 transcripts by qRT-PCR with a primer pair designed based on identical regions of TaATG6a, 6b, and 6c. Amplification of the wheat β-tubulin gene served as an internal control. CK is from BSMV:GFP-treated control plants and Arabic numbers indicate individual BSMV:ATG6-treated plants. (E) Constitutive and Blumeria graminis f. sp. tritici (Bgt)-induced autophagy are impaired in BSMV:ATG6-treated 92R137/Yangmai 1587 (Pm21) plants. The fourth leaves were excised from BSMV-treated plants at 0 and 7 days after inoculation (dai) with Bgt conidia, infiltrated with E-64d and then stained with LysoTracker Red. LysoTracker Red-stained punctate autolysosome-like structures are indicated by arrows. The results were reproduced from three independent experiments using three or more plants in each experiment. Scale bars represent 5 μm.

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