Skip to main content
Figure 2 | BMC Plant Biology

Figure 2

From: Wheat homologs of yeast ATG6 function in autophagy and are implicated in powdery mildew immunity

Figure 2

Characterization of wheat autophagy-related ATG6 sequences. (A) The genomic sequences of monocot ATG6s contain a CpG island covering the start codon. The CpG island was predicted using the web tool CpG Island Searcher (http://cpgislands.usc.edu/) with a CpG island standard of longer than 500 bp in length, a minimum G + C content of 55% and a minimum 0.65 ratio of the observed versus the expected presence of CpG dinucleotides. Gray, horizontal lines represent the 1600-bp start codon-covering genomic sequences of TaATG6b from wheat, OsATG6b (Os03g0644000) from rice and AtATG6 (At3g61710) from Arabidopsis. Short vertical red lines represent CpG dinucleotides. Blue horizontal thick lines represent the ranges of predicted CpG islands. (B) Short GC-rich stretch just downstream of the start codon in monocot ATG6 sequences. Sequences shown are the 5ʹ-terminal cDNA sequences beginning with the start codons of monocot ATG6s, including TaATG6a, 6b and 6c from wheat, AM075824 from Hordeum vulgare, NM_001050415, CT831793 and NM_001186426 from rice, XM_003569522 from Brachypodium distachyon, XM_006644464 from Oryza brachyantha, NM_001112413 from Zea mays, XM_002456829 and AM075825 from Sorghum bicolor, AM075826 from Saccharum officinarum, and XM_004971054 and XM_004969496 from Setaria italic. The GC-rich stretches (green background) are not properly aligned but the flanking sequences are aligned. The reading frames from ATG are indicated by the upper vertical lines.

Back to article page