Calibration of qPCR for quantification of
: (a) Standard curve analysis: Standard curve showing the correlation between the log10 DNA amounts (ng) vs. the Ct values for 10 fold dilution of P. cajani pure genomic DNA. (b) Melting curve analysis: The melting curve (SYBR Green florescence versus temperature) of specific amplifications from the ITS sequences of 5.8S rDNA at different concentrations. The single pick of targeted amplicon at melting temperature (Tm) 79.8°C indicates the specificity of the qPCR primers to P. cajani. No contaminating product was detected in PCR reaction.