Interactions with CDKA1 and CDKA2 proteins using pairwise Y2H co-transformation assays. Yeast PJ694-alpha cells were co-transformed with bait (DBD) and prey (AD) plasmid as indicated. Co-transformation was analyzed on medium lacking leucine and tryptophan (-L-T). Co-transformants were tested for their ability to activate the histidine marker gene by assessing yeast growth on medium lacking leucine, tryptophan and histidine (-L-T-H) and for their ability to activate the LacZ reporter gene (X-Gal). As a negative control, the GUS gene was used. For each combination, three independent colonies were screened, of which one is shown.