PCR amplification with the
specific primers. An agarose gel (1.2%) electrophoresis of PCR fragments amplified from the gene encoding LCB-1-P lyase (isogroup 000007) in U. vulgaris with different templates. 1–6: cDNA prepared from RNA extracted from two different plant individuals, 7: genomic DNA. NC: negative control with water instead of DNA. (A) PCR with exon-specific primers UV405_F1 and UV405_R1. (B). PCR with intron-specific primer UV405_F2 and exon-specific primer UV405_R1. Annealing temperature is indicated above the lanes. Standard of molecular weights is shown on the both sides of the gel.