Proteomic and metabolomic analyses provide insight into production of volatile and non-volatile flavor components in mandarin hybrid fruit

Yu, Qibin; Plotto, Anne; Baldwin, Elizabeth A; Bai, Jinhe; Huang, Ming; Yu, Yuan; Dhaliwal, Harvinder S; Gmitter, Frederick G

doi:10.1186/s12870-015-0466-9

Table 3 Differentially expressed proteins in fruit flesh of Temple (Te) versus Murcott (Mu) mandarin hybrid fruit

From: Proteomic and metabolomic analyses provide insight into production of volatile and non-volatile flavor components in mandarin hybrid fruit

Accession	Name	Species	iTRAQ ratio fold change
			Stage 1		Stage 2		Stage 3
			Te/Mu	P value	Te/Mu	P value	Te/Mu	P value
gi\|11596186	cystatin-like protein	Citrus x paradisi	4.041	0.036	0.647	0.002
gi\|118061963	extracellular solute-binding protein, family 5	Roseiflexus castenholzii DSM 13941					0.483	0.047
gi\|119367477	putative H-type thioredoxin	Citrus cv. Shiranuhi	10.782	0.001	0.404	0.001
gi\|119367479	putative cyclophilin	Citrus cv. Shiranuhi			0.588	0.037	2.347	0.002
gi\|121485004	cytosolic phosphoglycerate kinase	Helianthus annuus	5.535	0.002
gi\|124360080	Galactose mutarotase-like	Medicago truncatula					1.724	0.003
gi\|125546170	hypothetical protein OsI_14032	Oryza sativa Indica Group			0.561	0.014
gi\|14031067	dehydrin COR15	Citrus x paradisi					2.806	0.000
gi\|147809484	hypothetical protein	Vitis vinifera			0.608	0.022	0.696	0.065
gi\|147836508	hypothetical protein	Vitis vinifera					1.630	0.024
gi\|147853192	hypothetical protein	Vitis vinifera					1.803	0.018
gi\|15219028	26.5 kDa class I small heat shock protein-like	Arabidopsis thaliana					0.491	0.008
gi\|15235730	phosphoenolpyruvate carboxykinase (ATP), putative/PEP carboxykinase, putative/PEPCK, putative	Arabidopsis thaliana					1.899	0.034
gi\|159471948	U2 snRNP auxiliary factor, large subunit	Chlamydomonas reinhardtii			0.255	0.044
gi\|166850556	CTRSFT1-like protein	Poncirus trifoliata	3.261	0.011	0.237	0.005
gi\|169160465	phospholipase D alpha	Citrus sinensis	4.060	0.000	0.240	0.000	0.573	0.000
gi\|17225598	pyruvate decarboxylase	Fragaria x ananassa					0.286	0.012
gi\|183579873	chitinase	Citrus unshiu	1.534	0.012
gi\|192912988	40S ribosomal protein S4	Elaeis guineensis	1.601	0.049
gi\|218202932	14-3-3 protein	Dimocarpus longan			0.227	0.016
gi\|221327587	ascorbate peroxidase	Citrus maxima	4.863	0.000	0.180	0.049
gi\|2213425	hypothetical protein	Citrus x paradisi	0.627	0.000	0.524	0.001
gi\|223949137	unknown	Zea mays	5.116	0.003
gi\|224069008	predicted protein	Populus trichocarpa	6.992	0.001
gi\|224099429	predicted protein	Populus trichocarpa			0.587	0.014	0.316	0.002
gi\|224109966	predicted protein	Populus trichocarpa			0.476	0.040
gi\|224127346	predicted protein	Populus trichocarpa			0.156	0.007	0.641	0.043
gi\|224128794	predicted protein	Populus trichocarpa			0.298	0.007	0.382	0.022
gi\|224135985	predicted protein	Populus trichocarpa			0.248	0.006	0.366	0.021
gi\|225424861	PREDICTED: hypothetical protein isoform 2	Vitis vinifera					0.536	0.040
gi\|225425914	PREDICTED: hypothetical protein	Vitis vinifera			0.429	0.002	0.425	0.010
gi\|225439785	PREDICTED: hypothetical protein	Vitis vinifera			0.441	0.007	0.658	0.023
gi\|225441981	PREDICTED: hypothetical protein	Vitis vinifera			0.304	0.002	0.568	0.007
gi\|225442225	PREDICTED: hypothetical protein	Vitis vinifera	9.896	0.015	0.576	0.010	0.571	0.002
gi\|225451968	PREDICTED: similar to mangrin	Vitis vinifera	4.507	0.040	0.263	0.095
gi\|231586	ATP synthase subunit beta	Hevea brasiliensis			0.134	0.004	0.555	0.007
gi\|242199340	UDP-glucosyltransferase family 1 protein	Citrus sinensis	7.535	0.002	0.394	0.008	0.539	0.030
gi\|255539613	phosphoglucomutase, putative	Ricinus communis			0.142	0.020
gi\|255543156	conserved hypothetical protein	Ricinus communis	7.967	0.000
gi\|255544686	eukaryotic translation elongation factor, putative	Ricinus communis			0.424	0.006	0.323	0.008
gi\|255550111	heat-shock protein, putative	Ricinus communis	3.788	0.043
gi\|255551036	aspartate aminotransferase, putative	Ricinus communis					0.599	0.037
gi\|255561582	Patellin-3, putative	Ricinus communis			0.588	0.017
gi\|255571742	peptidase, putative	Ricinus communis			0.275	0.004
gi\|255586766	monodehydroascorbate reductase, putative	Ricinus communis			0.429	0.003	0.493	0.001
gi\|255641409	unknown	Glycine max			0.645	0.021
gi\|255642211	unknown	Glycine max	0.521	0.011	0.121	0.001
gi\|255644696	unknown	Glycine max	5.914	0.002
gi\|257659867	unnamed protein product	Linum usitatissimum			0.329	0.235	0.368	0.047
gi\|257675725	unnamed protein product	Zea mays	3.832	0.019
gi\|257690969	unnamed protein product	Citrus sinensis			0.384	0.002
gi\|257712573	unnamed protein product	Brassica napus	9.086	0.011			0.664	0.006
gi\|257720002	unnamed protein product	Glycine max			0.551	0.001	0.387	0.007
gi\|257726687	unnamed protein product	Zea mays	1.650	0.035	0.387	0.001
gi\|27462762	malate dehydrogenase	Lupinus albus					0.305	0.003
gi\|29124973	unknown	Populus tremuloides					2.039	0.031
gi\|33316389	valencene synthase	Citrus sinensis					25.730	0.022
gi\|33325127	eukaryotic translation initiation factor 5A isoform VI	Hevea brasiliensis					1.914	0.039
gi\|33340236	copper/zinc superoxide dismutase	Citrus limon	3.706	0.001	0.638	0.004
gi\|37524017	COR15	Citrus clementina x Citrus reticulata	10.311	0.006			2.382	0.010
gi\|3790102	pyrophosphate-dependent phosphofructokinase alpha subunit	Citrus x paradisi	1.724	0.025			0.554	0.011
gi\|40646744	mitochondrial citrate synthase precursor	Citrus junos			0.201	0.032	0.553	0.018
gi\|4580920	vacuole-associated annexin VCaB42	Nicotiana tabacum			0.209	0.046	0.330	0.007
gi\|4704605	glycine-rich RNA-binding protein	Picea glauca	4.452	0.009
gi\|530207	heat shock protein	Glycine max	4.177	0.045
gi\|544437	Probable phospholipid hydroperoxide glutathione peroxidase	Citrus sinensis					3.140	0.039
gi\|5764653	NADP-isocitrate dehydrogenase	Citrus limon			0.430	0.006	0.437	0.003
gi\|6094476	Thiazole biosynthetic enzyme	Citrus sinensis			0.228	0.007
gi\|6166140	Elongation factor 1-delta 1	Oryza sativa Japonica Group	7.427	0.045	0.654	0.028
gi\|6225526	Isopentenyl-diphosphate Delta-isomerase I	Clarkia breweri			0.562	0.033
gi\|624674	heat shock protein	Citrus maxima
gi\|624676	citrate synthase precursor	Citrus maxima	2.731	0.020
gi\|62900641	Plastid-lipid-associated protein	Citrus unshiu	6.082	0.002	0.289	0.000	0.662	0.022
gi\|63333659	beta-1,3-glucanase class III	Citrus clementina x Citrus reticulata			0.493	0.141	2.712	0.000
gi\|6518112	H + −ATPase catalytic subunit	Citrus unshiu	4.754	0.017	0.598	0.007
gi\|6682841	sucrose synthase	Citrus unshiu	3.194	0.025	0.632	0.009
gi\|6682843	sucrose synthase	Citrus unshiu			0.144	0.008	0.575	0.024
gi\|7024451	glycine-rich RNA-binding protein	Citrus unshiu	1.886	0.531
gi\|70609690	glutamate decarboxylase	Citrus sinensis	3.588	0.025	0.643	0.043
gi\|7269241	UDPglucose 4-epimerase-like protein	Arabidopsis thaliana	0.424	0.011	0.158	0.004
gi\|74486744	translation elongation factor 1A-9	Gossypium hirsutum	4.923	0.008
gi\|76573375	triosphosphate isomerase-like protein	Solanum tuberosum					0.311	0.000
gi\|77417715	SOD	Citrus maxima	0.638	0.017	0.118	0.010	0.322	0.013
gi\|77540216	triosephosphate isomerase	Glycine max					0.514	0.022
gi\|77744899	temperature-induced lipocalin	Citrus sinensis	4.028	0.018	0.548	0.016
gi\|82623427	glyceraldehyde 3-phosphate dehydrogenase-like	Solanum tuberosum			0.661	0.297
gi\|862480	valosin-containing protein	Glycine max	1.510	0.029	0.374	0.010
gi\|870794	polyubiquitin	Arabidopsis thaliana	4.534	0.005
gi\|90820120	UDP-glucose pyrophosphorylase	Cucumis melo	7.835	0.028
gi\|9082317	actin	Helianthus annuus	3.959	0.051	0.527	0.001
gi\|9280626	UDP-glucose pyrophosphorylase	Astragalus membranaceus	9.821	0.002			1.626	0.022
gi\|9757974	polyubiquitin	Arabidopsis thaliana			0.585	0.011

The P value was selected from the most significant one among three biological replications. Additional file 1: Table S2 has the result from all three biological replications. Stage 1 was on December 22, 2008, Stage 2 was on January 30, 2009, and Stage 3 was on March 11, 2009.

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