Figure 5
The localization of peroxidase activity in developing Scots pine seeds. (A, B) Oxidized DAB (brown colour) indicated peroxidase activity in the seed coat, nucellar layers, megagametophyte cells surrounding the corrosion cavity, suspensor cells and subordinate embryos at the early (A) and late (B) embryogeny. (C) The seed treated with the reaction buffer without DAB. asr = arrow-shaped region, e = embryo, esr = embryo surrounding region, m = megagametophyte, mm = megaspore membranes, nc = nucellar cap, nl = nucellar layers, nt = cellular nucellus, sc = seed coat, se = subordinate embryo, sr = suspensor remnants.